Effect of tumor-associated macrophages on lncRNA PURPL/miR-363/PDZD2 axis in osteosarcoma cells

Effect of tumor-associated macrophages on lncRNA PURPL/miR-363/PDZD2 axis in osteosarcoma cells

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Abstract Tumor-associated macrophages (TAMs) are known Deep Fryers to participate in osteosarcoma (OS) progression.As demonstrated in our previous research, miR-363 played a tumor inhibitory effect in OS cells via lowering the PDZ domain containing 2 (PDZD2) expression.The regulatory roles of TAMs on miR-363/PDZD2 and the internal mechanism relating to long noncoding RNA p53 upregulated regulator of P53 levels (lncRNA PURPL) are examined in this study.

TAM-like macrophages were formed by inducing CD14+ peripheral blood mononuclear cells (PBMCs).The TAMs migration was detected after MG-63 cells transfected with miR-363 mimics or inhibitors.We then analyzed the regulatory activity of PURPL on miR-363 expression.

We also tested the influences of PURPL overexpression/knockdown on MG-63 cell proliferation, migration, invasion, and epithelial-mesenchymal transition (EMT), as well as TAMs migration.Silence in PDZD2 expression was used to confirm the effects of PURPL on MG-63 cells.We successfully induced TAM-like macrophages.

MG-63 cells transfecting miR-363 mimics suppressed TAMs migration while transfecting a converse effect was seen in miR-363 inhibitor.TAMs raised PURPL expression in MG-63 cells, which was an upstream regulator of miR-363.Along with TAMs migration, PURPL overexpression promoted MG-63 cell proliferation, migration, invasion, and EMT.

An opposite influence was seen due to the PURPL knockdown.The Equipment silence of PDZD2 weakened the influences of PURPL overexpression on MG-63 cells and TAMs migration.On modulating the PURPL/miR-363/PDZD2 axis, TAMs-promoted OS development might be achieved.